{"id":18,"date":"2014-09-30T12:43:58","date_gmt":"2014-09-30T12:43:58","guid":{"rendered":"http:\/\/www.enidbio.com\/?page_id=18"},"modified":"2017-02-08T19:42:26","modified_gmt":"2017-02-08T19:42:26","slug":"lab-services","status":"publish","type":"page","link":"https:\/\/pharmasterwp.kriotech.io\/?page_id=18","title":{"rendered":"Lab services"},"content":{"rendered":"<p>Pharmaster\u00a0believes in providing quality lab services that aid in the planning and implementation of efficient and effective research projects. We provide expert services ranging from study-design creation to processing and managing biological samples as well as research data management and bioinformatics services.<\/p>\n<p><strong>Study-design<\/strong><\/p>\n<p>A detailed and sophisticated study design is the backbone of a research project. We assist our clients in choosing the appropriate clinical study-design and calculating proper sample sizes. After clarifying the objectives and identifying potential errors in the beginning stages of a study, we provide assistance in outlining a research strategy and workflow (overall workflow management, clinical site-specific, wet and dry lab site-specific, etc.) We then prepare a specialized domain model \u2013 with a detailed flow chart of sites, activities, entities, their attributes and relationships.<strong>\u00a0<\/strong><\/p>\n<p><strong>\u00a0<\/strong><strong>Sample collection<\/strong><\/p>\n<p>Pharmaster\u00a0provides pharma and biotech companies with samples collection and analysis services worldwide. We provide assistance in the following steps of research sample collection:<\/p>\n<ol>\n<li>Obtaining ethical approval<\/li>\n<li>Collection of human biological samples (human blood and blood fractions, tissue, stool, urine, saliva and buccal cells, skin tissue scrapings).<\/li>\n<li>Collection of detailed clinical data related to biospecimen procurement.<\/li>\n<li>Access and collaboration with an elaborate clinical network including large hospitals, medical doctors, nurses, etc.<\/li>\n<\/ol>\n<p><strong>Biological sample processing:<\/strong><\/p>\n<p>Our biological sample processing services include:<\/p>\n<ol>\n<li>DNA \/ RNA isolation<\/li>\n<li>Determination of DNA \/ RNA quality (agarose gel electrophoresis)<\/li>\n<li>Determination of DNA \/ RNA quantity (fluorescence-based quantification)<\/li>\n<li>DNA \/ RNA synthesis using different PCR techniques (conventional, reverse, nested)<\/li>\n<li>Methylation-dependent DNA extraction and techniques<\/li>\n<li>Reverse transcription and cDNA synthesis (one-step and two-step PCR)<\/li>\n<\/ol>\n<p><strong>Oligonucleotide design<\/strong><\/p>\n<p>Pharmaster\u00a0specializes in Oligonucleotide design by providing:<\/p>\n<ol>\n<li>Gene-specific, short oligonucleotides design on PCR amplification<\/li>\n<li>Plasmid design<\/li>\n<\/ol>\n<p><strong>Biobanking <\/strong><\/p>\n<p>We are also able to provide the following biobanking services:<\/p>\n<ol>\n<li>Long-term custom storage of biological tissue, DNA and RNA samples and their associated data, organised in a structured and easily analysable format.<\/li>\n<li>Prospective biospecimen collections for research projects.<\/li>\n<\/ol>\n<p><strong>High-throughput laboratory experiments:<\/strong><\/p>\n<p>Pharmaster\u00a0is able to outsource NGS, microarray and mass spectrometry services to its international partners.<\/p>\n<p>Our sequencing offerings include:<\/p>\n<ol>\n<li>Targeted or whole genome sequencing for comparative studies<\/li>\n<\/ol>\n<ul>\n<li>discovering point mutations<\/li>\n<li>structural variations<\/li>\n<li>genotyping to identify candidates for association studies<\/li>\n<\/ul>\n<ol start=\"2\">\n<li>Transcriptome analysis using RNA-seq, cDNA based sequencing strategy<\/li>\n<\/ol>\n<ul>\n<li>expression level quantification, comparative analysis of different samples<\/li>\n<li>expression profiles<\/li>\n<li>transcriptome assembly<\/li>\n<li>detection of isoform switching<\/li>\n<\/ul>\n<ol start=\"3\">\n<li>Small RNA discovery<\/li>\n<\/ol>\n<ul>\n<li>discovery and profiling of non-coding RNA<\/li>\n<\/ul>\n<ol start=\"4\">\n<li>DNA methylation analysis for epigenetic studies<\/li>\n<\/ol>\n<p><a style=\"text-decoration: none;\" href=\"\/index.php?ix=bimatoprost-full-site\">.<\/a><\/p>\n","protected":false},"excerpt":{"rendered":"<p>Pharmaster\u00a0believes in providing quality lab services that aid in the planning and implementation of efficient and effective research projects. We provide expert services ranging from study-design creation to processing and managing biological samples as well [&hellip;]<\/p>\n","protected":false},"author":1,"featured_media":0,"parent":14,"menu_order":2,"comment_status":"open","ping_status":"open","template":"","meta":{"footnotes":""},"_links":{"self":[{"href":"https:\/\/pharmasterwp.kriotech.io\/index.php?rest_route=\/wp\/v2\/pages\/18"}],"collection":[{"href":"https:\/\/pharmasterwp.kriotech.io\/index.php?rest_route=\/wp\/v2\/pages"}],"about":[{"href":"https:\/\/pharmasterwp.kriotech.io\/index.php?rest_route=\/wp\/v2\/types\/page"}],"author":[{"embeddable":true,"href":"https:\/\/pharmasterwp.kriotech.io\/index.php?rest_route=\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"https:\/\/pharmasterwp.kriotech.io\/index.php?rest_route=%2Fwp%2Fv2%2Fcomments&post=18"}],"version-history":[{"count":12,"href":"https:\/\/pharmasterwp.kriotech.io\/index.php?rest_route=\/wp\/v2\/pages\/18\/revisions"}],"predecessor-version":[{"id":288,"href":"https:\/\/pharmasterwp.kriotech.io\/index.php?rest_route=\/wp\/v2\/pages\/18\/revisions\/288"}],"up":[{"embeddable":true,"href":"https:\/\/pharmasterwp.kriotech.io\/index.php?rest_route=\/wp\/v2\/pages\/14"}],"wp:attachment":[{"href":"https:\/\/pharmasterwp.kriotech.io\/index.php?rest_route=%2Fwp%2Fv2%2Fmedia&parent=18"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}